Fig. 1 · Structure
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T4 DNA ligase
DNA Ligase
T4 DNA ligase is an ATP-dependent enzyme that functions as a DNA ligase, facilitating the repair of single-stranded nicks in double-stranded DNA and joining duplex DNA restriction fragments with both blunt and cohesive ends. This enzyme effectively seals adjacent 5′-phosphate and 3′-hydroxyl termini at single-stranded breaks, making it essential for various molecular biology applications, including cloning, recombinant DNA technology, and the construction of plasmid vectors. Due to its ability to ligate single-stranded DNA in the absence of duplex DNA, T4 DNA ligase is a versatile tool in genomic research and genetic engineering.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 9015-85-4 |
| Applications | DNA Ligase |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.