We use analytics and advertising cookies, which you can accept or reject; see our privacy policy.

Current choice:

Fig. 1 · Structure
Cat. No.
A11119
CAS No.
130-61-0
Formula
C21H26N2S2.HCl
Mol. Weight
407

Documents & Downloads

Thioridazine hydrochloride

Cat. No. A11119 CAS 130-61-0
Thioridazine is a dopamine receptor antagonist that displays antipsychotic activity. Recently thioridazine is also reported to successfully kill human cancer stem cells.

In Stock

Notify me when the price drops

For laboratory research use only. We do not sell to patients.

Free Aliquoting for Your Order

Tell us your preferred amount per vial. We’ll confirm the options.

Biological Activity

Target & Activity

Target Activity Value
serotonin  

In Vitro Studies

Thioridazine at 15 μM reduced cell viability in HeLa and HEC-1-A cells. In GBM8401 cells treated with Thioridazine for 14 days, the clonogenic IC50 was <10 μM. [1,2]

Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.

Thioridazine reduces HeLa cell viability

Cell-based study · HeLa [1]

In HeLa cells, treatment with Thioridazine at 15 μM reduced cell viability.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
cell viabilityReported result were reduced by treatment of thioridazine[1]

Literature-Reported Treatment Conditions

Treatment concentration
15 μM
Reported limits
Not stated in the sentences used: route, schedule, vehicle, control.
Methods and source locations

Compound reported in the paper: Thioridazine

The paper names Thioridazine without stating the salt or form; not verified as the hydrochloride form supplied as A11119. No dose conversion or lot validation.

Materials and methods > Cell viability assays (S8); Results > Thioridazine regulates the cell proliferation and apoptosis (S55, S56); Fig. 1 legend (S94)

cell viability: cell viability — Results > Thioridazine regulates the cell proliferation and apoptosis (S56)

Clonogenic response to thioridazine in glioblastoma cells

Cell-based study · GBM8401 cells [2]

GBM8401 cells were treated with Thioridazine at 1 to 10 μM for 14 days. The clonogenic IC50 was <10 μM.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
Clonogenic IC50 (μM)Clonogenic IC50 <10 μM[2]

Literature-Reported Treatment Conditions

Treatment concentration
1 to 10 μM
Exposure duration
cells were treated for 14 days. The culture medium was changed on days 4, 8 and 12, and fresh drugs were added at this time.
Reported limits
Not stated in the sentences used: route, vehicle, control.
Methods and source locations

Compound reported in the paper: Thioridazine

The paper names Thioridazine without stating the salt or form; not verified as the hydrochloride form supplied as A11119. No dose conversion or lot validation.

Materials and Methods > Clonogenic assay (S108, S109, S112); Figure 1 legend (S181); Table 2 (T2.h2, T2.r11)

Clonogenic IC50 (μM): Clonogenic IC50 (μM) — Table 2 (T2.h2, T2.r11)

Thioridazine reduces endometrial cell viability

Cell-based study · HEC-1-A [1]

In HEC-1-A cells, treatment with Thioridazine at 15 μM reduced cell viability.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
cell viabilityReported result were reduced by treatment of thioridazine[1]

Literature-Reported Treatment Conditions

Treatment concentration
15 μM
Reported limits
Not stated in the sentences used: route, schedule, vehicle, control.
Methods and source locations

Compound reported in the paper: Thioridazine

The paper names Thioridazine without stating the salt or form; not verified as the hydrochloride form supplied as A11119. No dose conversion or lot validation.

Materials and methods > Cell viability assays (S8); Results > Thioridazine regulates the cell proliferation and apoptosis (S55, S56); Fig. 1 legend (S94)

cell viability: cell viability — Results > Thioridazine regulates the cell proliferation and apoptosis (S56)

In Vivo Studies

Wild-type male mice received Thioridazine hydrochloride at 25 mg/kg by intraperitoneal injection. There was no significant difference in average litter size between thioridazine-treated and control sires. [3]

Average litter size following thioridazine treatment in mice

Animal study · Wild-type 129S6 mice; wild-type male mice [3]

Wild-type male mice received Thioridazine hydrochloride at 25 mg/kg by intraperitoneal injection. There was no significant difference in average litter size between thioridazine-treated and control sires.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
average litter sizeReported result There was no significant difference in average litter size between thioridazine-treated and control sires[3]

Conditions shared by the study groups

Route
intraperitoneal injection
Treatment course
every 3 days starting at 21 days of age for 3 weeks (7 doses total)
Vehicle
sterile 0.9% NaCl
Study groups and reported outcomes
Group / doseComparatorObserved resultReference
Thioridazine hydrochloride 25 mg/kgan equal volume of sterile 0.9% NaCl in the same mannerThere was no significant difference in average litter size between thioridazine-treated and control sires[3]
Thioridazine hydrochloride 25 mg/kg
Model: Wild-type 129S6 mice; wild-type male mice
Methods and source locations

Compound reported in the paper: Thioridazine hydrochloride; Compound label in the paper: Thioridazine

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

2. Materials and Methods > 2.16. Fertility Study (S104, S105, S106); 3. Results > 3.4. Thioridazine Treatment of Mice Bearing Human EC Cell Xenografts Extends Survival (S185, S188)

average litter size: average litter size; comparator: an equal volume of sterile 0.9% NaCl in the same manner — 3. Results > 3.4. Thioridazine Treatment of Mice Bearing Human EC Cell Xenografts Extends Survival (S188)

Thioridazine hydrochloride 25 mg/kg · 2. Materials and Methods > 2.16. Fertility Study (S105); 3. Results > 3.4. Thioridazine Treatment of Mice Bearing Human EC Cell Xenografts Extends Survival (S188)

Specifications

Manufacturer Adooq Bioscience
Actions Antagonist
CAS Number 130-61-0
Formula C21H26N2S2.HCl
Molecular Weight 407
SMILES
CN1CCCCC1CCN2C3=CC=CC=C3SC4=C2C=C(C=C4)SC.Cl

Storage & Solubility

In Vitro Solubility

Reported Solubility

SolventReported Solubility
DMSO 75 mg/mL (184.25 mM)

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Research References

  1. [1] Kang S, Dong SM, Kim BR, et al. Thioridazine induces apoptosis by targeting the PI3K/Akt/mTOR pathway in cervical and endometrial cancer cells. Apoptosis (2012). PMID: 22460505 DOI: 10.1007/s10495-012-0717-2 Source material
  2. [2] Cheng HW, Liang YH, Kuo YL, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis (2015). PMID: 25950483 DOI: 10.1038/cddis.2015.77 Source material
  3. [3] Loehr AR, Pierpont TM, Gelsleichter E, et al. Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors. Cancers (Basel) (2021). PMID: 33922599 DOI: 10.3390/cancers13092045 Source material

Added to your cart

View Cart Checkout
Loading...