Fig. 1 · Structure

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ZnAF-2

Biochemical Assay Reagent

ZnAF-2 is a high-affinity fluorescence probe specifically designed for Zn2+ detection, featuring a dissociation constant (Kd) of 2.7 nM. This cell-impermeable compound displays minimal baseline fluorescence, with a notable 51-fold enhancement in fluorescence intensity upon stoichiometric binding to Zn2+. ZnAF-2 demonstrates negligible affinity for other metal ions such as Ca2+, Mg2+, Na+, or K+, making it ideal for investigating zinc-related biological processes and biochemical assays.

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Specifications

Manufacturer Adooq Bioscience
CAS Number 321859-11-4
Applications Biochemical Assay Reagent
Formula C34H28N4O5
Molecular Weight 572.61
SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(NCCN(CC6=NC=CC=C6)CC7=NC=CC=C7)=C5

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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