4-Nitrophenyl β-D-Cellobioside is a cellotriose analog primarily targeted by β-glucosidases, including TxGH116 and ThCel7B. This compound serves as a substrate in enzymatic assays to evaluate cellulase activity through the hydrolysis process, resulting in the release of p-nitrophenol (PNP). Its utility in research applications includes the study of cellulose degradation and enzyme kinetics, contributing valuable insights into carbohydrate metabolism and enzyme specificity.
4-Nitrophenyl β-D-Cellobioside is a cellotriose analog primarily targeted by β-glucosidases, including TxGH116 and ThCel7B. This compound serves as a substrate in enzymatic assays to evaluate cellulase activity through the hydrolysis process, resulting in the release of p-nitrophenol (PNP). Its utility in research applications includes the study of cellulose degradation and enzyme kinetics, contributing valuable insights into carbohydrate metabolism and enzyme specificity.
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