4-Nitrophenyl β-D-cellotrioside is a chromogenic substrate specifically designed for endoglucanases and cellulose biohydrolases. This compound serves as a fluorescent dye, undergoing hydrolysis by enzymes to release 4-nitrophenol, which results in a distinct yellow color. The enzymatic activity can be quantitatively assessed by measuring the absorbance change at 405 nm, making it a useful tool for studying cellulose degradation and enzyme kinetics in various biological research applications.
4-Nitrophenyl β-D-cellotrioside is a chromogenic substrate specifically designed for endoglucanases and cellulose biohydrolases. This compound serves as a fluorescent dye, undergoing hydrolysis by enzymes to release 4-nitrophenol, which results in a distinct yellow color. The enzymatic activity can be quantitatively assessed by measuring the absorbance change at 405 nm, making it a useful tool for studying cellulose degradation and enzyme kinetics in various biological research applications.
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