Fig. 1 · Structure
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4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside
Detection Substrate
4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside is a specific substrate for the detection of chitinase enzymes. Through its cleavage by chitinases, it generates detectable fluorescence, facilitating the study of chitinase activity in various biological systems. This reagent is widely used in research applications focusing on enzyme activity assays and carbohydrate metabolism.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 7284-16-4 |
| Applications | Detection Substrate |
| Formula | C22H31N3O13 |
| Molecular Weight | 545.49 |
| Synonyms | pNP-chitobiose |
| SMILES | OC[C@@H](O[C@H]1OC(C=C2)=CC=C2[N](=O)=O)[C@H]([C@@H]([C@H]1NC(C)=O)O)O[C@H](O[C@@H]3CO)[C@@H]([C@H]([C@@H]3O)O)NC(C)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.