5(6)-Carboxyfluorescein

Catalog No.: A21120

5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein Chemical Structure

CAS NO. 72088-94-9

5(6)-Carboxyfluorescein contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.

Availability: In stock

Package Price Qty
500 mg

Regular Price: $45.00

Special Price $36.00

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Biological Activity

5(6)-Carboxyfluorescein contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 26.57 mL 132.87 mL 265.73 mL
0.5 mM 5.31 mL 26.57 mL 53.15 mL
1 mM 2.66 mL 13.29 mL 26.57 mL
5 mM 0.53 mL 2.66 mL 5.31 mL

*The above data is based on the productmolecular weight 376.32. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Catalog Num A21120
CAS No. 72088-94-9
Formula C21H12O7
M. Wt 376.32
Purity >98%
Synonyms 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers
SMILES OC1=CC=C(C2(O3)C(C=C(C(O)=O)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(C(O)=O)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
Storage

Store lyophilized at -20ºC, keep desiccated.
In lyophilized form, the chemical is stable for 36 months.
In solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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