Fig. 1 · Structure
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5-Bromo-3-indolyl β-D-galactopyranoside
β-galactosidase Substrate
5-Bromo-3-indolyl β-D-galactopyranoside is a chromogenic substrate specifically designed for β-galactosidase. Upon hydrolysis by the enzyme, it generates a 5-bromoindole intermediate that is oxidized to produce an insoluble blue precipitate, facilitating visual detection. This substrate effectively recognizes bacterial β-galactosidases, including those derived from the lacZ gene of Escherichia coli, and operates optimally at pH 7.4. Its applications include histochemical detection of reporter gene expression in transgenic organisms, enabling the localization of β-galactosidase activity in various tissues, such as mouse embryos and muscle samples.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 97753-82-7 |
| Applications | β-galactosidase Substrate |
| Formula | C14H16BrNO6 |
| Molecular Weight | 374.18 |
| Synonyms | Bluo-Gal |
| SMILES | BrC1=CC=C2C(C(O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@@H]3O)CO)=CN2)=C1 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.