Fig. 1 · Structure
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5-Bromo-6-chloro-3-indolyl phosphate p-toluidine
Sugar
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine is a substrate for alkaline phosphatase, facilitating the visualization of enzyme activity. Upon hydrolysis by alkaline phosphatase, it generates an insoluble magenta product, which serves as a colorimetric indicator. This reagent is widely used in various biological research applications, including histochemistry and enzyme assays, to assess alkaline phosphatase activity in cells and tissues.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 6769-80-8 |
| Applications | Sugar |
| Formula | C15H15BrClN2O4P |
| Molecular Weight | 433.62 |
| Synonyms | Magenta phosphate |
| SMILES | ClC1=CC2=C(C=C1Br)C(OP(O)(O)=O)=CN2.NC3=CC=C(C)C=C3 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.