5-Ethynyluridine is a cell-permeable nucleoside that enables the labeling of newly synthesized RNA. Its primary applications include the isolation and sequencing of nascent RNA from neuronal populations in vivo, making it valuable for studying transcriptional changes in nervous system disease models. Additionally, 5-Ethynyluridine features an alkyne group that facilitates copper-catalyzed azide-alkyne cycloaddition (CuAAc), allowing for efficient conjugation with azide-containing molecules in click chemistry experiments.
5-Ethynyluridine is a cell-permeable nucleoside that enables the labeling of newly synthesized RNA. Its primary applications include the isolation and sequencing of nascent RNA from neuronal populations in vivo, making it valuable for studying transcriptional changes in nervous system disease models. Additionally, 5-Ethynyluridine features an alkyne group that facilitates copper-catalyzed azide-alkyne cycloaddition (CuAAc), allowing for efficient conjugation with azide-containing molecules in click chemistry experiments.
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