Ac-Ile-Glu-Thr-Asp-pNA is a substrate specifically designed for caspase-8. It features the Ile-Glu-Thr-Asp (IETD) peptide sequence, which is efficiently recognized and cleaved by caspase-8, resulting in the release of p-nitroaniline. The subsequent increase in p-nitroaniline concentration can be quantitatively measured through colorimetric detection at 405 nm, providing a reliable assay for evaluating caspase-8 enzymatic activity in various biological research applications.
Ac-Ile-Glu-Thr-Asp-pNA is a substrate specifically designed for caspase-8. It features the Ile-Glu-Thr-Asp (IETD) peptide sequence, which is efficiently recognized and cleaved by caspase-8, resulting in the release of p-nitroaniline. The subsequent increase in p-nitroaniline concentration can be quantitatively measured through colorimetric detection at 405 nm, providing a reliable assay for evaluating caspase-8 enzymatic activity in various biological research applications.
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