Fig. 1 · Structure
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AF488 NHS ester
Fluorescence Probe
AF488 NHS ester is an amine-reactive fluorescence probe with an emission maximum of 525 nm. This compound specifically reacts with amines and sulfhydryl groups in aqueous and biological samples, leading to a change in chemical structure and fluorescence properties upon derivatization. It is widely used in a variety of biological research applications, including labeling proteins and biomolecules for fluorescence microscopy and flow cytometry.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 1374019-99-4 |
| Applications | Fluorescence Probe |
| Formula | C25H17N3O13S2 |
| Molecular Weight | 631.54 |
| SMILES | O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(ON5C(CCC5=O)=O)=O)=C1)O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.