Alkyne-phenol is a clickable probe specifically designed for use with ascorbate peroxidase 2 (APEX2). This reagent significantly enhances APEX-labeling efficiency in intact yeast cells due to its improved permeability through cell walls compared to traditional APEX2 substrates. Alkyne-phenol aids in the identification of APEX-labeling sites, providing clear insights into the membrane topology of mitochondrial proteins. Additionally, it features an alkyne group that allows for copper-catalyzed azide-alkyne cycloaddition (CuAAc), making it a versatile tool for click chemistry applications in biological research.
Alkyne-phenol is a clickable probe specifically designed for use with ascorbate peroxidase 2 (APEX2). This reagent significantly enhances APEX-labeling efficiency in intact yeast cells due to its improved permeability through cell walls compared to traditional APEX2 substrates. Alkyne-phenol aids in the identification of APEX-labeling sites, providing clear insights into the membrane topology of mitochondrial proteins. Additionally, it features an alkyne group that allows for copper-catalyzed azide-alkyne cycloaddition (CuAAc), making it a versatile tool for click chemistry applications in biological research.
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