In MDCK cells, Amantadine as a single agent had a cytotoxic concentration of 37–40 µg/mL. In Huh7 cells pretreated before SARS-CoV-2 pseudovirus infection, Amantadine inhibited CTSL enzyme activity and blocked infection-induced CTSL activation compared with vehicle. In GFP‐LC3‐transduced HCT116 cells assessed with and without Amantadine, the amantadine-sensitive IAV strain completely failed to relocalise LC3 in its presence. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Amantadine cytotoxicity in MDCK cells
Cell-based study · MDCK cells [1]
In MDCK cells, Amantadine added as a single agent had a TC50 of 37–40 µg/mL.
Literature-Reported Treatment Conditions
- Treatment concentration
- 0, 0.001, 0.0032, 0.01, 0.032, 0.1, 0.32, 1, 3.2, 10, 32 and 100 µg/mL
- Method
- added to MDCK cells in 96-well microplates
- Reported limits
- Not stated in the sentences used: schedule, vehicle.
Methods and source locations
Compound reported in the paper: Amantadine
The paper names Amantadine without stating the salt or form; not verified as the hydrochloride form supplied as A10060. No dose conversion or lot validation.
Materials and Methods > Cell-Based Assays (S15, S16, S17, S19); Materials and Methods > EC50, TC50, and Synergy Calculations (S28); Results > Cytotoxicity of Antiviral Drugs as Single Agents and in Double and Triple Combinations (S131)
50% cytotoxic concentration (TC50): 50% cytotoxic concentration (TC50); comparator: uninfected cells (cell controls) — Materials and Methods > Cell-Based Assays (S19); Results > Cytotoxicity of Antiviral Drugs as Single Agents and in Double and Triple Combinations (S131)
Amantadine and CTSL activity in Huh7 cells
Cell-based study · Huh7 cells; n = 7 [2]
Huh7 cells were pretreated with 300 μM Amantadine for 1 h before SARS-CoV-2 pseudovirus infection. Compared with vehicle, Amantadine inhibited CTSL enzyme activity and blocked infection-induced CTSL activation.
Literature-Reported Treatment Conditions
- Treatment concentration
- 300 μM
- Exposure duration
- for 1 h and were then infected with SARS-CoV-2 pseudovirus
- Method
- pretreated
- Co-treatment
- Other interventions in this design: SARS-CoV-2 pseudovirus 1.3 × 104 TCID50/ml. Compound effect isolated by the comparison: vehicle.
- Reported limits
- Not stated in the sentences used: vehicle.
Methods and source locations
Compound reported in the paper: Amantadine
The paper names Amantadine without stating the salt or form; not verified as the hydrochloride form supplied as A10060. No dose conversion or lot validation.
Results > CTSL inhibitors prevent SARS-CoV-2 pseudovirus infection in vitro (S103, S104, S105, S111)
CTSL enzyme activity in Huh7 cells: CTSL enzyme activity in Huh7 cells; comparator: vehicle — Results > CTSL inhibitors prevent SARS-CoV-2 pseudovirus infection in vitro (S103, S104, S111)
Amantadine and LC3 relocalisation in infected HCT116 cells
Cell-based study · GFP‐LC3‐transduced HCT116 cells [3]
Amantadine was added after infection in GFP‐LC3‐transduced HCT116 cells. In the comparison of its presence or absence, the amantadine-sensitive IAV strain completely failed to relocalise LC3 in the presence of Amantadine.
Literature-Reported Treatment Conditions
- Exposure duration
- Amantadine was added 3 h postinfection. Samples were fixed at 16 h postinfection
- Method
- added
- Co-treatment
- Other interventions in this design: . Compound effect isolated by the comparison: in the presence or absence of amantadine.
- Reported limits
- Not stated in the sentences used: dose, vehicle.
Methods and source locations
Compound reported in the paper: Amantadine
The paper names Amantadine without stating the salt or form; not verified as the hydrochloride form supplied as A10060. No dose conversion or lot validation.
Results > Influenza A infection activates non‐canonical autophagy via a proton channel (S109, S110); Figure 8 legend (S266, S267, S268)
Confocal images of GFP‐LC3‐transduced HCT116 cells: Confocal images of GFP‐LC3‐transduced HCT116 cells; comparator: absence of amantadine — Results > Influenza A infection activates non‐canonical autophagy via a proton channel (S110); Figure 8 legend (S266)
