Bis-Tris is an amine buffer effective for protein and nucleic acid systems, functioning within a pH range of 5.8 to 7.2. It can be combined with HEPES and cacodylic acid to achieve a pH of 8, facilitating the monitoring of fluorescence emission intensity at 305 nm. Additionally, gels prepared with Bis-Tris minimize protein degradation in samples maintained at higher pH levels (pH 8.5), making it a valuable reagent for various biochemical assays and analytical applications.
Bis-Tris is an amine buffer effective for protein and nucleic acid systems, functioning within a pH range of 5.8 to 7.2. It can be combined with HEPES and cacodylic acid to achieve a pH of 8, facilitating the monitoring of fluorescence emission intensity at 305 nm. Additionally, gels prepared with Bis-Tris minimize protein degradation in samples maintained at higher pH levels (pH 8.5), making it a valuable reagent for various biochemical assays and analytical applications.
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