Fig. 1 · Structure
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Chlorophenol red-β-D-galactopyranoside
β-Galactosidase Substrate
Chlorophenol red-β-D-galactopyranoside is a specific chromogenic substrate for β-galactosidase, facilitating quantitative colorimetric assays. Initially appearing pale yellow, it undergoes hydrolysis by β-galactosidase to release chlorophenol red, resulting in a color shift to purple-red in alkaline or neutral pH environments. This chromatic change can be accurately measured at wavelengths of 540-572 nm using visible spectrophotometry, making it a valuable tool in various research applications involving β-galactosidase activity.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 99792-79-7 |
| Applications | β-Galactosidase Substrate |
| Formula | C25H22Cl2O10S |
| Molecular Weight | 585.41 |
| Synonyms | CPRG |
| SMILES | O=S1(OC(C2=CC(Cl)=C(C=C2)O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)(C4=CC(Cl)=C(C=C4)O)C5=CC=CC=C51)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.