Fig. 1 · Structure

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Coelenteramine 400a hydrochloride

RLuc Substrate

Coelenteramine 400a hydrochloride serves as a substrate for Renilla luciferase (RLuc), facilitating the emission of blue light at 395 nm. This compound enhances bioluminescence reactions by substituting the sulfur and oxygen heteroatoms of the methylene bridge, resulting in improved signal resolution. Coelenteramine 400a hydrochloride is applicable in studies of bioluminescence resonance energy transfer (BRET), making it a valuable reagent for bioluminescence research.

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Specifications

Manufacturer Adooq Bioscience
Actions Substrate
CAS Number 2320429-05-6
Applications RLuc Substrate
Formula C26H22ClN3O
Molecular Weight 427.93
Synonyms Coelenterazine 400a hydrochloride; Bisdeoxycoelenterazine hydrochloride
SMILES
O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31.[H]Cl

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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