Fig. 1 · Structure

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Coelenteramine 400a

RLuc Substrate

Coelenteramine 400a is a highly effective substrate for Renilla luciferase (RLuc), facilitating the emission of blue light at 395 nm. This compound enhances bioluminescence signal resolution by replacing sulfur and oxygen heteroatoms in the methylene bridge, resulting in distinct color changes during RLuc bioluminescence reactions. Coelenteramine 400a is particularly valuable in research applications involving bioluminescence resonance energy transfer (BRET), enabling advanced studies in cell signaling and molecular interactions.

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Specifications

Manufacturer Adooq Bioscience
Actions Substrate
CAS Number 70217-82-2
Applications RLuc Substrate
Formula C26H21N3O
Molecular Weight 391.46
Synonyms Coelenterazine 400a; Bisdeoxycoelenterazine
SMILES
O=C1C(CC2=CC=CC=C2)=NC3=C(NC(C4=CC=CC=C4)=CN31)CC5=CC=CC=C5

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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