Copper Fluor-4 (CF4) is a highly selective fluorescent probe for Cu+ ions, utilizing a rhodol dye scaffold. With a Kd value of 2.9×10−13 M, it demonstrates exceptional selectivity for copper over competing metal ions such as zinc and iron, as well as common cellular alkali and alkaline earth metals. Copper Fluor-4 remains stable within a physiologically relevant pH range of 6 to 8, making it suitable for various biological applications, including imaging and quantification of copper ions in live cells. Excitation occurs at 415 nm with emission at 660 nm.
Copper Fluor-4 (CF4) is a highly selective fluorescent probe for Cu+ ions, utilizing a rhodol dye scaffold. With a Kd value of 2.9×10−13 M, it demonstrates exceptional selectivity for copper over competing metal ions such as zinc and iron, as well as common cellular alkali and alkaline earth metals. Copper Fluor-4 remains stable within a physiologically relevant pH range of 6 to 8, making it suitable for various biological applications, including imaging and quantification of copper ions in live cells. Excitation occurs at 415 nm with emission at 660 nm.
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