In E. coli MG1655 resuspended in LB containing 3M D-Sorbitol for 20 min at room temperature, mean glycan strain lengths and peptidoglycan subunit eluent percentages were the same across treatments. In HEK293 cells stably expressing wild-type γ2, incubation with 1.5 M D-Sorbitol for 1 hr clearly increased Thr-172 phosphorylation without changing α1/α2 subunit expression. D-Sorbitol treatment also caused a small increase in ADP:ATP in both WT and RG cells. In C. difficile VPI 10463, medium supplementation with 5000 mg/L D-Sorbitol decreased growth lag compared with controls without additional carbohydrate. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Glycan lengths and peptidoglycan subunit profiles in E. coli
Cell-based study · E. coli MG1655 [1]
E. coli MG1655 was resuspended in LB containing 3M D-Sorbitol and held at room temperature for 20 min. Mean glycan strain lengths and percentages of various peptidoglycan subunit eluents were the same across all treatments.
Literature-Reported Treatment Conditions
- Treatment concentration
- 3M
- Exposure duration
- room temperature for 20 min after resuspension
- Method
- resuspended
- Vehicle
- LB
Methods and source locations
Compound reported in the paper: D-Sorbitol; Compound label in the paper: Sorbitol
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Methods > Analysis of peptidoglycan composition (S80, S81, S82); Extended Data Figure 2 legend (S174, S175)
Mean glycan strain lengths (i) and percentage of various peptidoglycan subunit eluents (ii): Mean glycan strain lengths (i) and percentage of various peptidoglycan subunit eluents (ii); comparator: untreated — Methods > Analysis of peptidoglycan composition (S81, S82); Extended Data Figure 2 legend (S175)
Phosphorylation response in wild-type cells
Cell-based study · HEK293 cells stably expressing wild-type γ2 (referred to below as WT cells) [2]
HEK293 cells stably expressing wild-type γ2 were incubated with 1.5 M D-Sorbitol for 1 hr. Western blotting showed clear increases in Thr-172 phosphorylation without changes in α1/α2 subunit expression.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1.5 M
- Exposure duration
- 1 hr
- Method
- incubated
- Reported limits
- Not stated in the sentences used: vehicle, control.
Methods and source locations
Compound reported in the paper: D-Sorbitol; Compound label in the paper: Sorbitol
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Characterization of Stably Transfected Cells (S3, S4); Results > Phosphorylation of AMPK (S30, S31); Figure 4 legend (S143)
Thr-172 phosphorylation assessed by Western blotting: Thr-172 phosphorylation assessed by Western blotting — Results > Phosphorylation of AMPK (S30, S31); Figure 4 legend (S143)
Growth lag in C. difficile
Cell-based study · C. difficile VPI 10463 [3]
C. difficile VPI 10463 grown in medium supplemented with 5000 mg/L D-Sorbitol showed decreased growth lag compared with controls without additional carbohydrate.
Literature-Reported Treatment Conditions
- Treatment concentration
- 5000 mg/L
- Method
- media supplemented with glucose, fructose, mannitol, or sorbitol
- Reported limits
- Not stated in the sentences used: schedule, vehicle.
Methods and source locations
Compound reported in the paper: D-Sorbitol; Compound label in the paper: Sorbitol
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > A metabolic environment that favors C. difficile germination and growth (S48); Methods > C. difficile in vitro growth curves with carbohydrates (S212, S213)
growth lag: growth lag; comparator: controls without additional carbohydrate — Results > A metabolic environment that favors C. difficile germination and growth (S48); Methods > C. difficile in vitro growth curves with carbohydrates (S212)
ADP:ATP response to osmotic stress
Cell-based study · HEK293 cells stably expressing wild-type γ2 (referred to below as WT cells) [2]
HEK293 cells stably expressing wild-type γ2 were incubated with 1.5 M D-Sorbitol for 1 hr. Osmotic stress using sorbitol caused a small increase in ADP:ATP in both WT and RG cells.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1.5 M
- Exposure duration
- 1 hr
- Method
- incubated
- Reported limits
- Not stated in the sentences used: vehicle.
Methods and source locations
Compound reported in the paper: D-Sorbitol; Compound label in the paper: Sorbitol
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Characterization of Stably Transfected Cells (S3, S4); Results > Changes in Cellular Nucleotides (S39); Figure 4 legend (S143); Figure 5 legend (S144, S145, S147)
Contents of ATP and ADP in perchloric acid extracts were estimated using capillary electrophoresis and are expressed as a ratio: Contents of ATP and ADP in perchloric acid extracts were estimated using capillary electrophoresis and are expressed as a ratio; comparator: controls performed at the same time without treatment — Results > Changes in Cellular Nucleotides (S39); Figure 4 legend (S143); Figure 5 legend (S144, S145)
