Fig. 1 · Structure
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D-Val-Leu-Lys-pNA dihydrochloride
Plasmin/Plasminogen Substrate
D-Val-Leu-Lys-pNA dihydrochloride is a chromogenic peptide substrate specifically designed for plasmin and plasminogen. It serves as a sensitive substrate for the DFE27 serine protease from Bacillus subtilis DC27. Upon catalysis by plasmin, D-Val-Leu-Lys-pNA dihydrochloride undergoes hydrolysis to release p-nitroaniline (pNA), enabling quantitative measurement of fibrinolytic activity via colorimetric detection at 405 nm. This reagent is essential for studies involving fibrinolysis and related processes.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 62354-43-2 |
| Applications | Plasmin/Plasminogen Substrate |
| Formula | C23H40Cl2N6O5 |
| Molecular Weight | 551.51 |
| Synonyms | D-Val-Leu-Lys-p-itoailide dihydrochloride; D-VLK-pNA dihydrochloride; S 2251 dihydrochloride |
| SMILES | CC([C@H](C(N[C@H](C(N[C@H](C(NC1=CC=C([N+]([O-])=O)C=C1)=O)CCCCN)=O)CC(C)C)=O)N)C.Cl.Cl |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.