Fig. 1 · Structure
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DiSBAC10
Fluorochrome
DiSBAC10 is a voltage-sensitive fluorochrome designed to monitor electrical activity across cell membranes in Förster Resonance Energy Transfer (FRET) assays. When cells are in a polarized state, DiSBAC10 localizes to the outer leaflet of the membrane, facilitating energy transfer from excited fluorescein-labeled proteins, resulting in photon re-emission at a longer wavelength. Upon cell depolarization, DiSBAC10 translocates to the inner leaflet, increasing the spatial separation between fluorophores and thereby decreasing the FRET signal, making it an essential tool for studying membrane potential dynamics.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 169211-45-4 |
| Applications | Fluorochrome |
| Formula | C51H88N4O4S2 |
| Molecular Weight | 885.40 |
| SMILES | O=C1/C(C(N(CCCCCCCCCC)C(N1CCCCCCCCCC)=S)=O)=C\C=C\C2C(N(CCCCCCCCCC)C(N(CCCCCCCCCC)C2=O)=S)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.