Fig. 1 · Structure
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Eriochrome black T, Indicator
Metal Ion Indicators
Eriochrome Black T is a metal ion indicator primarily targeting Ca2+ and Mg2+ ions. This complexing agent is widely utilized in complexometric titrations, where it forms colored complexes with metal ions, signaling the endpoint through a noticeable color change. Additionally, Eriochrome Black T serves as an anionic azo dye in photocatalytic degradation studies, allowing for the assessment of photocatalyst efficiency. In loop-mediated isothermal amplification (LAMP), the dye's color transitions from purple to sky blue upon the consumption of Mg2+, indicating a positive reaction, with an optimal concentration of 60 μM for effective detection of Mycobacterium tuberculosis at a sensitivity of 1 pg DNA per reaction.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 1787-61-7 |
| Applications | Metal Ion Indicators |
| Formula | C20H12N3NaO7S |
| Molecular Weight | 461.38 |
| SMILES | O=S(C1=C2C=C([N+]([O-])=O)C=CC2=C(/N=N/C3=CC=C4C=CC=CC4=C3O)C(O)=C1)(O[Na])=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.