Fig. 1 · Structure
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Ethidium homodimer
DNA Fluorochrome
Ethidium homodimer is a high-affinity fluorescent nucleic acid dye targeting DNA and RNA. It exhibits enhanced fluorescence upon binding, with an increase of over 30 times, making it an effective tool for detecting nucleic acids in various biological samples, including mammalian cells, bacteria, yeast, and fungi. Due to its positive charge, Ethidium homodimer is unable to penetrate live cell membranes; however, it can access the nuclei of dead cells, resulting in the emission of red fluorescence. This characteristic makes it suitable for applications involving cell viability and nucleic acid detection, with an excitation/emission wavelength of 528/617 nm.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 61926-22-5 |
| Applications | DNA Fluorochrome |
| Formula | C46H50Cl4N8 |
| Molecular Weight | 856.75 |
| Synonyms | EthD-1 |
| SMILES | NC1=CC=C2C(C(C3=CC=CC=C3)=[N+](CCCNCCNCCC[N+]4=C(C5=CC=CC=C5)C6=CC(N)=CC=C6C7=C4C=C(N)C=C7)C8=C2C=CC(N)=C8)=C1.Cl.Cl.[Cl-].[Cl-] |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.