Ethidium monoazide bromide is a DNA intercalating fluorescent dye specifically targeting nucleic acids in cells with compromised membranes. Upon photoactivation, this compound covalently binds to DNA, enabling the selective staining of dead cells. Additionally, it serves as a versatile click chemistry reagent, containing an azide group that can participate in copper-catalyzed azide-alkyne cycloaddition (CuAAc) or strain-promoted alkyne-azide cycloaddition (SPAAC) with alkyne-functionalized molecules. Ethidium monoazide bromide is valuable in microbiological studies and cellular viability assays.
Ethidium monoazide bromide is a DNA intercalating fluorescent dye specifically targeting nucleic acids in cells with compromised membranes. Upon photoactivation, this compound covalently binds to DNA, enabling the selective staining of dead cells. Additionally, it serves as a versatile click chemistry reagent, containing an azide group that can participate in copper-catalyzed azide-alkyne cycloaddition (CuAAc) or strain-promoted alkyne-azide cycloaddition (SPAAC) with alkyne-functionalized molecules. Ethidium monoazide bromide is valuable in microbiological studies and cellular viability assays.
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