Fig. 1 · Structure
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Hippuryl-His-Leu-OH
ACE Substrate
Hippuryl-His-Leu-OH is a specific substrate for angiotensin-converting enzyme (ACE I), utilized as a molecular tool for detecting ACE activity in vitro. It undergoes hydrolysis by ACE, releasing hippuric acid (HA), which serves as a quantitative measure of ACE activity and aids in screening for ACE inhibitors. The release of His-Leu can further react with o-phthalaldehyde or Fluorescamine for fluorescence detection. This reagent is applicable in the in vitro evaluation of ACE inhibitors related to hypertension and cardiovascular diseases, and contributes to studies on ACE activity alterations in various physiological and pathological conditions, including renal compensatory hypertrophy.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 31373-65-6 |
| Applications | ACE Substrate |
| Formula | C21H27N5O5 |
| Molecular Weight | 429.47 |
| Synonyms | N-Benzoyl-Gly-His-Leu |
| SMILES | CC(C)C[C@H](NC(=O)[C@H](Cc1cnc[nH]1)NC(=O)CNC(=O)c1ccccc1)C(O)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.