GNAQ-associated differences in uveal melanoma cell viability
Cell-based study · Eleven author-designated uveal melanoma cell lines: three GNAQ codon-209 mutant and eight wild-type lines. [1]
After 72 hours, mutant lines were more sensitive in the MTS assay. The authors reported IC50 values of 2–4 µM in mutant lines versus 8 µM or greater in wild-type lines. This non-isogenic panel supports an association with genotype, rather than proving genotype alone determines response. Some panel lines share patient origin.
| Endpoint | Reported result | Reference |
|---|---|---|
| MTS viability in GNAQ-mutant versus wild-type cell lines | IC50 Mutant: 2–4 µM; wild type: 8 µM or greater | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- Concentration series spanning 0–10 µM, as shown in Figure 1B.
- Exposure duration
- 72 hours
- Method
- MTS cell viability assay; standard culture medium contained 10% FBS.
- Reported limits
- Carrier identity/concentration for the Figure 1 single-agent series is not explicitly specified; salt form and lot are not specified.
Methods and source locations
Compound reported in the paper: Enzastaurin (LY317615); Compound label in the paper: ENZ
Published experiments used Lilly-provided enzastaurin with unspecified salt form; results do not validate an Adooq lot.
Results, first subsection; Figure 1A–B; Methods, Cell Lines, Cell viability assay and Statistical Methods
MTS viability in GNAQ-mutant versus wild-type cell lines: MTS; Figure 1 reports mean ± SD of percent viability from three independent experiments; 0 µM reference condition. — Results, first subsection; Figure 1A–B and caption; Methods, Cell viability assay