Nitro blue tetrazolium chloride

Catalog No.: A21323

Dehydrogenases substrate

Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride Chemical Structure

CAS NO. 298-83-9

Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.

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250 mg

Regular Price: $54.00

Special Price $43.20

500 mg

Regular Price: $108.00

Special Price $86.40

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Biological Activity

Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 12.23 mL 61.15 mL 122.3 mL
0.5 mM 2.45 mL 12.23 mL 24.46 mL
1 mM 1.22 mL 6.12 mL 12.23 mL
5 mM 0.24 mL 1.22 mL 2.45 mL

*The above data is based on the productmolecular weight 817.64. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Catalog Num A21323
Actions Substrate
CAS No. 298-83-9
Formula C40H30Cl2N10O6
M. Wt 817.64
Purity >98%
Synonyms NBT
SMILES COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
Storage

Store lyophilized at -20ºC, keep desiccated.
In lyophilized form, the chemical is stable for 36 months.
In solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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