Fig. 1 · Structure

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Nitrocefin

Cat. No. A46317 CAS 41906-86-9

β-lactamase Substrate

Nitrocefin is a chromogenic substrate specifically targeting β-lactamase enzymes. It displays a characteristic color shift from yellow to red upon hydrolysis of the β-lactam ring, enabling visual quantification of β-lactamase activity. This reagent is widely utilized in studies of enzyme inhibition and the development of β-lactamase-resistant antibiotics.

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Specifications

Manufacturer Adooq Bioscience
Actions Substrate
CAS Number 41906-86-9
Applications β-lactamase Substrate
Formula C21H16N4O8S2
Molecular Weight 516.50
SMILES
O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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