Serine Protease

Serine protease inhibitors act on peptidases that cleave proteins with a serine, histidine and aspartate triad. The family spans digestive enzymes such as trypsin and chymotrypsin, coagulation factors, kallikreins and neutrophil elastase. Phenylmethylsulfonyl fluoride (PMSF), 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF HCl) and the polypeptide aprotinin are broad inhibitors often added to lysis buffers. Sivelestat sodium salt and alvelestat inhibit neutrophil elastase, which is also covered by the neighboring Elastases category. Camostat mesylate and nafamostat mesylate act on transmembrane serine protease 2 (TMPRSS2) and related trypsin-like enzymes. In 2020, camostat mesylate was reported to block TMPRSS2-dependent cell entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

13 Items

  • D-Leu-Thr-Arg-pNA
    Cat. No.
    A77673

    D-Leu-Thr-Arg-pNA

    D-Leu-Thr-Arg-pNA is a specific substrate for γ-Tryptase, enabling the quantitative assessment of γ-Tryptase activity in biochemical assays. This compound is valuable for studying the inhibition effects of small molecule inhibitors on γ-Tryptase, facilitating research in areas such as allergic responses and inflammation. Its application in enzyme activity assays makes it essential for exploring therapeutic interventions related to protease-mediated diseases.

    5 mg
    $55.00
    10 mg
    $90.00
    All 4 sizes
  • AAA-pNA
    Cat. No.
    A77689

    AAA-pNA

    AAA-pNA is a chromogenic substrate specifically designed for the enzyme Tripeptidyl-peptidase II. This compound enables the quantification of Tripeptidyl-peptidase II activity through colorimetric analysis, facilitating studies on enzyme kinetics and functionality. It is useful in various research applications, including investigations into proteolytic processes and the role of Tripeptidyl-peptidase II in biological systems.

    5 mg
    $65.00
    10 mg
    $105.00
    All 4 sizes
  • Ac-Gly-Lys-OMe acetate
    Cat. No.
    A77697

    Ac-Gly-Lys-OMe acetate

    Ac-Gly-Lys-OMe acetate is a substrate for urokinase, facilitating the study of its enzymatic activity. This compound is essential for assessing the effects of small molecule inhibitors on urokinase functionality, providing valuable insights in biochemical and pharmacological research related to fibrinolysis and tissue remodeling.

    5 mg
    $45.00
    10 mg
    $70.00
    All 5 sizes
  • Suc-AAPY-pNA
    Cat. No.
    A77699

    Suc-AAPY-pNA

    Suc-AAPY-pNA is an oligopeptide compound that serves as a substrate for various proteases. It undergoes hydrolysis at the peptide bond between tyrosine and p-nitroaniline, resulting in the release of p-nitroaniline, which can be quantified by its absorption peak at OD410. This reagent is utilized in preclinical assays to assess the activity of acidic, neutral, and alkaline proteases, making it valuable for studies on proteolytic processes.

    Out of stock

    All 3 sizes
  • Boc-Phe-Ser-Arg-AMC
    Cat. No.
    A77715

    Boc-Phe-Ser-Arg-AMC

    Boc-Phe-Ser-Arg-AMC is a trypsin substrate featuring a Boc protecting group. Upon cleavage by trypsin, this peptide substrate releases the fluorescent compound AMC, enabling the measurement of trypsin activity through fluorescence. It is an effective tool for studying trypsin function and kinetics in various biochemical assays and research applications.

    Out of stock

    All 3 sizes
  • Ala-Phe-Pro-pNA
    Cat. No.
    A77716

    Ala-Phe-Pro-pNA

    Ala-Phe-Pro-pNA is a chromogenic substrate specifically designed for tripeptidyl peptidase. This substrate allows for the assessment of tripeptidyl peptidase activity by producing a colorimetric change upon cleavage. Its properties make it a valuable tool for studying protease functions and for applications in biochemical assays involving tripeptidyl peptidases.

    25 mg
    $1,360.00
    50 mg
    $1,790.00
    All 3 sizes
  • Ac-KQL-AMC
    Cat. No.
    A77720

    Ac-KQL-AMC

    Ac-KQL-AMC is a fluorogenic substrate designed to quantify Trypsin-like activity. Upon cleavage by the proteasome, it produces a detectable fluorescence, making it a valuable tool for studying proteolytic activities in various biological contexts. This compound is particularly useful in research applications focused on protein degradation and turnover mechanisms.

    Out of stock

    All 3 sizes
  • Fluorescent Substrate for Subtillsin
    Cat. No.
    A77734

    Fluorescent Substrate for Subtillsin

    Fluorescent Substrate for Subtilisin is designed to specifically detect the enzymatic activity of subtilisin, a bacterial serine protease. This substrate provides a sensitive and quantifiable method to evaluate subtilisin activity in various biological assays. It is suitable for applications in enzyme activity studies and protease-related research.

    Out of stock

    All 3 sizes
  • Boc-Ala-Gly-Pro-Arg-AMC
    Cat. No.
    A77746

    Boc-Ala-Gly-Pro-Arg-AMC

    Boc-Ala-Gly-Pro-Arg-AMC is a fluorogenic substrate designed for monitoring protease activity, particularly within serine proteases. This substrate is ideal for assessing enzymatic function in research applications involving protease activity, such as studying the oligopeptide enzyme B of Trypanosoma brucei. Its fluorescence allows for sensitive detection of enzymatic events, making it a valuable tool in biochemical and molecular biology studies.

    Out of stock

    All 3 sizes
  • Ala-Phe-Pro-βNA
    Cat. No.
    A77747

    Ala-Phe-Pro-βNA

    Ala-Phe-Pro-βNA is a substrate for prolyl tripeptidyl aminopeptidase derived from Porphyromonas gingivalis. This compound exhibits potent binding affinity toward H-Ala-Ile-pyrrolidin-2-yl boronic acid, demonstrating Ki values of 88.1 nM for PTP39 and 48.8 nM for the E636A mutant. It is valuable for research applications involving enzyme activity assays and studies of proteolytic processes.

    Out of stock

    All 3 sizes
  • 2-thioacetyl MAGE
    Cat. No.
    A77756

    2-thioacetyl MAGE

    2-thioacetyl MAGE is a colorimetric substrate specifically designed for the serine hydrolase enzyme KIAA1363. This compound facilitates the assessment of KIAA1363 enzymatic activity, enabling researchers to study its biological functions and implications in various cellular processes. Its application in biochemical assays contributes to the understanding of KIAA1363's role in health and disease.

    Out of stock

    All 3 sizes
  • Abz-AFRFSQ-EDDnp
    Cat. No.
    A77772

    Abz-AFRFSQ-EDDnp

    Abz-AFRFSQ-EDDnp is a substrate specifically designed for human kallikrein 6, exhibiting a catalytic efficiency of Kcat/Km = 38667 s-1 mM-1. This compound facilitates the study of kallikrein 6 activity and is instrumental in various biological assays aimed at exploring protease functionalities and related pathways. Its application is essential in research focused on cancer, neurodegenerative diseases, and other physiological processes where kallikrein 6 plays a pivotal role.

    Out of stock

    All 3 sizes
  • Ac-Gly-Lys-OMe
    Cat. No.
    A77784

    Ac-Gly-Lys-OMe

    Ac-Gly-Lys-OMe is a substrate for urokinase, primarily utilized to evaluate the enzymatic activity of this serine protease. This reagent is essential for investigating the effects of small molecule inhibitors on urokinase function in various biochemical assays and research applications focused on fibrinolysis and related pathways.

    25 mg
    $1,275.00
    50 mg
    $1,625.00
    All 3 sizes

13 Items

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