Fig. 1 · Structure

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Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone

Peroxidase Enzymes Chromogenic Substrate

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) is a chromogenic substrate specifically designed for peroxidase enzymes. Upon reaction with peroxidase and peroxide, PCNPH generates a distinct purple indamine dye, facilitating the visualization and quantification of enzymatic activity. This reagent is widely used in various biochemical assays and research applications involving oxidative stress and enzyme kinetics.

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Specifications

Manufacturer Adooq Bioscience
CAS Number 70421-66-8
Applications Peroxidase Enzymes Chromogenic Substrate
Formula C12H10N4O2
Molecular Weight 242.23
Synonyms PCNPH
SMILES
O=[N+](C1=CC=C(NN=CC2=NC=CC=C2)C=C1)[O-]

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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