In cultured T cells, Rotenone alone at higher doses could transiently induce mitochondrial ROS. Rotenone treatment significantly reduced cancer cell invasion in the 4T1 and B16F10 models. In mitochondria isolated from Control-HCT 116 p53−/− cells, Rotenone treatment increased H2O2 release. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Transient mitochondrial ROS induction in T cells
Cell-based study · T cells [1]
In cultured T cells, Rotenone alone at higher doses transiently induced mitochondrial ROS.
Literature-Reported Treatment Conditions
- Exposure duration
- for either 5 hr or 6 days
- Method
- cultured in the indicated amounts of rotenone
- Reported limits
- Not stated in the sentences used: dose, vehicle.
Methods and source locations
Compound reported in the paper: Rotenone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
RESULTS > ROS drives exhaustion by acting as a phosphatase inhibitor (S80); Extended Data Figure 6. legend (S269)
MitoSOX staining of T cells, normalized to control: MitoSOX staining of T cells, normalized to control; comparator: control — RESULTS > ROS drives exhaustion by acting as a phosphatase inhibitor (S80); Extended Data Figure 6. legend (S269)
Reduced cancer cell invasion
Cell-based study · 4T1 [2]
In 4T1 cells, Rotenone treatment significantly reduced invasion.
Literature-Reported Treatment Conditions
- Method
- rotenone treatment
- Reported limits
- Not stated in the sentences used: dose, schedule, vehicle, control.
Methods and source locations
Compound reported in the paper: Rotenone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > PGC-1α expression determines invasive capacity and metastasis potential of cancer cells (S50, S53); Material and Methods > Invasion and migration assays (S164)
invasion: invasion — Results > PGC-1α expression determines invasive capacity and metastasis potential of cancer cells (S50, S53)
H2O2 release from isolated mitochondria
Cell-based study · mitochondria isolated from Control-HCT 116 p53−/− cells [3]
Rotenone treatment increased H2O2 release from mitochondria isolated from Control-HCT 116 p53−/− cells. H2O2 levels were measured using Amplex Red.
Literature-Reported Treatment Conditions
- Treatment concentration
- 500 nM
- Method
- Mitochondria were treated
- Reported limits
- Not stated in the sentences used: schedule, vehicle.
Methods and source locations
Compound reported in the paper: Rotenone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results (S93, S96, S97, S98); Materials and methods > ROS measurements (S183, S184)
H2O2 levels were measured using Amplex Red: H2O2 levels were measured using Amplex Red; comparator: left untreated — Results (S93, S97, S98)
Reduced cancer cell invasion
Cell-based study · B16F10 [2]
In B16F10 cells, Rotenone treatment significantly reduced invasion.
Literature-Reported Treatment Conditions
- Method
- rotenone treatment
- Reported limits
- Not stated in the sentences used: dose, schedule, vehicle, control.
Methods and source locations
Compound reported in the paper: Rotenone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > PGC-1α expression determines invasive capacity and metastasis potential of cancer cells (S50, S53); Material and Methods > Invasion and migration assays (S164)
invasion: invasion — Results > PGC-1α expression determines invasive capacity and metastasis potential of cancer cells (S50, S53)
