Fig. 1 · Structure
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TMB dihydrochloride x.hydrate
Chromogenic Agent
TMB dihydrochloride x.hydrate serves as a chromogenic agent, primarily utilized in enzyme detection systems involving horseradish peroxidase (HRP). This non-toxic, non-mutagenic, and colorless dye demonstrates key biological activity by acting as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), effectively reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to yield blue oxidation products. Additionally, TMB dihydrochloride x.hydrate is capable of detecting singlet oxygen generated by the outer membrane of Escherichia coli ATCC 25922, making it valuable for various biochemical research applications.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 207738-08-7 |
| Applications | Chromogenic Agent |
| Formula | C16H22Cl2N2.xH2O |
| Molecular Weight | 349.3 |
| Synonyms | Sure Blue TMB dihydrochloride x.hydrate |
| SMILES | NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.Cl.Cl.O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.