Fig. 1 · Structure
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TMB dihydrochloride
Chromogenic Agent
TMB dihydrochloride is a chromogenic agent primarily utilized in enzyme detection systems involving horseradish peroxidase (HRP). This non-toxic, non-mutagenic dye undergoes oxidation in the presence of reactive oxygen and nitrogen species (ROS/RNS), yielding blue products that can be quantitatively measured. Additionally, TMB dihydrochloride serves as a photoacoustic probe, effectively detecting singlet oxygen in microbial samples, such as Escherichia coli ATCC 25922, thus supporting research in oxidative stress and cellular signaling pathways.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 64285-73-0 |
| Applications | Chromogenic Agent |
| Formula | C16H22Cl2N2 |
| Molecular Weight | 313.27 |
| Synonyms | BM blue dihydrochloride; Sure Blue TMB dihydrochloride |
| SMILES | NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[2HCl] |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.