Fig. 1 · Structure
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TMB monosulfate
Chromogenic Agent
TMB monosulfate is a chromogenic agent primarily utilized in enzyme detection systems that involve horseradish peroxidase (HRP). This non-toxic and non-mutagenic colorless dye effectively reacts with reactive oxygen and nitrogen species (ROS/RNS), including hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide, generating distinct blue oxidation products. Additionally, TMB monosulfate serves as a photoacoustic probe, demonstrating the capability to detect singlet oxygen produced by Escherichia coli ATCC 25922, making it valuable for various biological and biochemical research applications.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 54827-18-8 |
| Applications | Chromogenic Agent |
| Formula | C16H22N2O4S |
| Molecular Weight | 338.42 |
| Synonyms | BM blue monosulfate; Sure Blue TMB monosulfate |
| SMILES | NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.O=S(O)(O)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.