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Hoechst 33258

Name: Hoechst 33258
Brand: Adooq Bioscience
SKU: A21316
Availability: InStock

Cat. No. A21316 CAS 23491-44-3

fluorescent dye

Hoechst 33258 is a fluorescent dye that emits blue fluorescence when bound to dsDNA.

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Biological Activity

Cellular Effect

Cell Line	Activity	Value	Description	Reference
A2780	IC50	12 μM	Antitumor activity of compound for 96-h exposure in A2780 human ovarian cell line	[PMID: 11170623]
A2780cisR	IC50	9.5 μM	Antitumor activity of compound for 96-h exposure in A2780cisR (acquired resistance to cisplatin) human ovarian cell line	[PMID: 11170623]
A549	IC50	≥ 31.2 μg/mL	Cytotoxicity against human A549 cells assessed as reduction in cell survival after 24 hrs by resazurin assay	[PMID: 27301676]
AGS	GI50	112 x 10	Cytotoxicity against human AGS cells after 72 hrs by MTT assay	[PMID: 21227551]
BEAS-2B	IC50	≥ 31.2 μg/mL	Cytotoxicity against human BEAS2B cells assessed as reduction in cell survival after 24 hrs by resazurin assay	[PMID: 27301676]
Caco-2	GI50	84 x 10	Cytotoxicity against human Caco2 cells after 72 hrs by MTT assay	[PMID: 21227551]
CH1	IC50	26.5 μM	Antitumor activity of compound for 96-h exposure in CH1 human ovarian cell line	[PMID: 11170623]
HeLa	GI50	115.7 x 10	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 21227551]
HeLa	IC50	51.31 μM	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 22959518]
HEp-2	GI50	132.5 x 10	Cytotoxicity against human Hep2 cells after 72 hrs by MTT assay	[PMID: 21227551]
HL-60	IC50	32.43 μM	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 22959518]
MCF7	GI50	5.7 x 10	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 21227551]
MDCK	GI50	157.5 x 10	Cytotoxicity against MDCK cells after 72 hrs by MTT assay	[PMID: 21227551]
MIA PaCa-2	GI50	191.5 x 10	Cytotoxicity against human MIAPaCa2 cells after 72 hrs by MTT assay	[PMID: 21227551]
NCI-H358	GI50	91.7 x 10	Cytotoxicity against human NCI-H358 cells after 72 hrs by MTT assay	[PMID: 21227551]
SK-OV-3	IC50	> 100 μM	Antitumor activity of compound for 96-h exposure in SKOV-3 human ovarian cell line	[PMID: 11170623]
U-937	IC50	15.42 μM	Cytotoxicity against human U937 cells after 72 hrs by MTT assay	[PMID: 22959518]

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Specifications

Manufacturer	Adooq Bioscience
CAS Number	23491-44-3
Applications	fluorescent dye
Formula	C₂₅H₂₄N₆O
Molecular Weight	424.5
SMILES	OC1=CC=C(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)C=C1

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.