DNA synthesis in human periodontal ligament stem cells
Cell-based study · Cultured human periodontal ligament stem cells isolated from healthy extracted teeth [1]
Y-27632 increased EdU incorporation versus untreated cells. This endpoint indicates DNA synthesis and does not establish apoptosis protection or preserved differentiation.
| Endpoint | Reported result | Reference |
|---|---|---|
| EdU-positive nuclei as percentage of DAPI-positive nuclei, ordered by Y-27632 concentration | EdU incorporation 0 μM: 33.73 ± 2.06%; 5 μM: 44.70 ± 3.53%; 10 μM: 50.3 ± 2.42%; 20 μM: 49.55 ± 2.43%; 40 μM: 43.85 ± 2.37% (mean ± SEM) | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 0, 5, 10, 20 or 40 μM
- Exposure duration
- 24 h treatment; 2 h EdU labeling
- Method
- Addition to DMEM containing 10% FBS
- Co-treatment
- No additional experimental drug
- Reported limits
- Stock solvent not specified in the read assay methods. Methods describe five separate EdU experiments; the final Figure 1 caption n = 3 statement follows the colony panel, so a single definitive EdU n is not assigned.
Methods and source locations
Compound reported in the paper: Y-27632; Compound label in the paper: Y-27632 dihydrochloride
Evidence for the named dihydrochloride in cultured human PDLSCs; no Adooq lot-specific validation.
Materials and methods: Cell proliferation assay; Results: Y-27632 enhanced the proliferation of PDLSCs; Figure 1B–C and caption
EdU-positive nuclei as percentage of DAPI-positive nuclei, ordered by Y-27632 concentration: EdU labeling after 24 h Y-27632 exposure in DMEM with 10% FBS; 2 h labeling. Figure 1B–C; source text supplies exact values. All treated groups exceeded untreated control; 10/20 μM comparisons P < 0.001. No exact values estimated from bars. — Materials and methods: Cell proliferation assay; Results: Y-27632 enhanced the proliferation of PDLSCs; Figure 1B–C and caption