Y-27632 2HCl

Name: Y-27632 2HCl
Brand: Adooq Bioscience
SKU: A11001
Rating: 5 (49 reviews)

Catalog No.: A11001

ROCK Inhibitor

Adooq's Y-27632 2HCl has been cited by 49 publications

Y-27632 is a Rho-Associated Coil Kinase (ROCK) inhibitor that increases the cloning efficiency of human embryonic stem cells (hESCs).

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Y-27632 2HCl 146986-50-7, 129830-38-2 ROCK Inhibitor

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Grouped product items
Size	Price	Stock
5mg	$30.00	In stock
10mg	$50.00	In stock
25mg	$95.00	In stock
50mg	$165.00	In stock
100mg	$280.00	In stock
200mg	$450.00	In stock
500mg	$745.00	In stock
*10mM 1mL in DMSO**	$35.00	In stock
Bulk Size	Bulk Discount

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Data Sheet SDS

Other Forms of Y-27632 2HCl

Y-27632

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Adooq Products cited in reputable paper

Adooq's Y-27632 2HCl has been cited by 49 publications

Marcos Abreu Costa, .et al. , Genet Med, 2025, Aug 21;27(11):101562 PMID: 40852795
Miguel L Agoncillo, .et al. , Eur J Pharmacol, 2025, Jun 15:997:177465 PMID: 40054721
Peilin Li, .et al. , Ecotoxicol Environ Saf, 2024, Dec:288:117330 PMID: 39571255
Takaaki Fujimura, .et al. , NPJ Precis Oncol, 2024, Nov 17;8(1):264 PMID: 39551860
Ayumi Horikawa, .et al. , In Vitro Cell Dev Biol Anim, 2024, Nov 15 PMID: 39546193
Giovanni Tosi, .et al. , Nat Commun, 2024, Sep 18;15(1):8214 PMID: 39294175
Christina Zarouchlioti, .et al. , EBioMedicine, 2024, Oct:108:105328 PMID: 39278108
Kyunghyun Park, .et al. , Advanced Therapeutics, 2024, 7(6)
Maude Hamilton, .et al. , Biol Open, 2024, Jan 15; 13(1): bio059919 PMID: 38252116
Oliver Podmanicky, .et al. , Hum Mol Genet, 2024, Mar 1; 33(5): 435-447 PMID: 37975900
Masayuki Fukumoto, .et al. , PLoS One, 2024, Dec 5;19(12):e0313312 PMID: 39636897
Johannes Robert Fleischer, .et al. , Mol Cancer, 2023, Jan 24;22(1):17 PMID: 36691028
Vignesh Jayarajan, .et al. , Cells, 2023, Jan 17;12(3):346 PMID: 36766688
Giulia Bastianello, .et al. , Cell Rep, 2023, Dec 26;42(12):113555 PMID: 38088930
Charlotte Phelps, .et al. , Sci Rep, 2023, Oct 23;13(1):18062 PMID: 37872186
Mathilde Meyenberg, .et al. , Sci Rep, 2023, Aug 26;13(1):13964 PMID: 37633982
Yu-Ting Wu, .et al. , J Biomed Sci, 2023, Aug 21;30(1):70 PMID: 37605213
Heming Ning, .et al. , In Vitro Cell Dev Biol Anim, 2023, Jun;59(6):410-419 PMID: 37405627
Eric Lian, .et al. , STAR Protoc, 2023, May 22;4(2):102314 PMID: 37220001
Stephanie T Pohl, .et al. , Methods Mol Biol, 2023, 2584:371-387 PMID: 36495461
Charlotte Phelps, .et al. , Am J Physiol Cell Physiol, 2023, Mar 1;324(3):C787-C797 PMID: 36689673
Shunsuke Murakami, .et al. , Regen Ther, 2022, Nov 25;21:574-583 PMID: 36475025
Sergio Pedraza-Arevalo, .et al. , STAR Protoc, 2022, Aug 30;3(3):101656 PMID: 36092820
Ji-Hyun Lee, .et al. , Cell Stem Cell, 2022, May 5; 29(5): 826-839 PMID: 35523142
Adam Pietrobon, .et al. , Cell Rep, 2022, Jul 5;40(1):111048 PMID: 35793620
Sarkawt Hamad, .et al. , Stem Cell Res Ther, 2022, Jun 11;13(1):251 PMID: 35690874
Stasia Krishtul, .et al. , Polym Adv Technol, 2022, 33( 11): 3842-3852
Peter T Ruane, .et al. , Hum Reprod, 2022, Apr 1;37(4):777-792 PMID: 35079788
Takayuki Miyoshi, .et al. , J Gastroenterol, 2022, Jun;57(6):441-452 PMID: 35294680
Ana-Maria Cujba, .et al. , Cell Rep, 2022, Mar 1;38(9):110425 PMID: 35235779
Dan Zhao, .et al. , Poult Sci, 2022, Mar; 101(3): 101642 PMID: 35016046
Antigoni Gogolou, .et al. , Curr Protoc, 2021, Jun;1(6):e137 PMID: 34102038
Tiago De Oliveira, .et al. , Cancers (Basel), 2021, Mar 2;S2451-9456(21)00095-7 PMID: 33671096
Denise Muller, .et al. , BMC Med, 2021, Nov 16;19(1):300 PMID: 34781947
Ayumi Horikawa, .et al. , PloS one, 2021, Jul 12;16(7):e0254373 PMID: 34252142
Hayato Mizuta, .et al. , Nat Commun, 2021, Feb 24;12(1):1261 PMID: 33627640
Scott Bell, .et al. , Stem Cell Reports, 2021, Jul 13;16(7):1749-1762 PMID: 34214487
Shenyang Li, .et al. , J Cell Mol Med, 2020, Dec 20;25(2):1207-1220 PMID: 33342057
Takao FUCHIGAMI, .et al. , J Appl Oral Sci, 2020, 28: e20190558 PMID: 32348439
Jing-Yu Lin, .et al. , Cell Discov, 2020, 6: 20 PMID: 32284878
Yu Huang, .et al. , Stem Cells Int, 2019, 2019: 3975689 PMID: 31565060
XinYan, .et al. , Stem Cell Res, 2019, May 17 PMID: 31141763
Daniela F. Duarte Campos, .et al. , J Biomed Mater Res A, 2019, April 22 PMID: 31012205
Scott Bell, .et al. , Am J Hum Genet, 2019, May 2;104(5):815-834 PMID: 31031012
Ooi J, .et al. , Cell Rep, 2019, Feb 26;26(9):2494-2508 PMID: 30811996
Scott Bell, .et al. , Stem Cell Reports, 2018, Jul 10; 11(1):183-196 PMID: 29937144
Christina Zarouchlioti, .et al. , Am J Hum Genet, 2018, Apr 5; 102(4): 528-539 PMID: 29526280
Ai Takemoto, .et al. , Sci Rep, 2017, 7: 42186 PMID: 28176852
Scott Bell, .et al. , Stem Cells Transl Med, 2017, Mar; 6(3): 886-896 PMID: 28170165

Biological Activity

Description

Y-27632 is a Rho-Associated Coil Kinase (ROCK) inhibitor that increases the cloning efficiency of human embryonic stem cells (hESCs).

Targets

Target	Value

References

Narumiya et al (2000) Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325 273 PMID: 11036610

Uehata et al (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase. Nature 389 990 PMID: 9353125

Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters. 32 516 PMID: 22227712

Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282 PMID: 24172903

Wilson et al (2016) Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition. Tissue.Eng.Part C.Methods 22 1085 PMID: 27846787

Sato et al (2015) SnapShot: Growing Organoids from Stem Cells. Cell 161 1700 PMID: 26091044

Mahe et al (2014) Establishment of gastrointestinal epithelial organoids Curr.Protoc.Mouse.Biol 3 217 PMID: 25105065

Bartfeld et al (2015) In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 148 126 PMID: 25307862

Lancaster et al (2015) Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat. Protoc. 9 2329 PMID: 25188634

Jung et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 17 1225 PMID: 21892181

Wimmer et al (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565 505 PMID: 30651639

Ishizaki et al (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol.Pharmacol. 57 976 PMID: 10779382

Hu et al (2015) Direct conversion of normal and Alzheimer's Disease human fibroblasts into neuronal cells by small molecules. Cell Stem Cell 17 204 PMID: 26253202

Chan et al et al (2016) Development of bipotent cardiac/skeletal myogenic progenitors from MESP1+ mesoderm. Stem Cell Rep. 6 26 PMID: 26771351

Santos et al (2019) Urothelial organoids originating from Cd49f^high mouse stem cells display Notch-dependent differentiation capacity. Nat.Commun. 10 4407 PMID: 31562298

Schafer (2023) An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 186 2111 PMID: 37172564

Product Information

Catalog Num	A11001
Formula	C14H21N3O.2HCl
Molecular Weight	320.3
CAS Number	146986-50-7, 129830-38-2
SMILES	C[C@H](C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl
Storage	Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Solubility

In vitro (25°C)	DMSO	Warmed: 61 mg/mL (190.46 mM)
	Water	13 mg/mL (40.59 mM)
	Ethanol	Insoluble
In vivo	saline	9 mg/mL
* <1 mg/ml means slightly soluble or insoluble. * Please note that Adooq tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Preparing Stock Solutions

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.22 mL	156.1 mL	312.21 mL
0.5 mM	6.24 mL	31.22 mL	62.44 mL
1 mM	3.12 mL	15.61 mL	31.22 mL
5 mM	0.62 mL	3.12 mL	6.24 mL

Useful Calculator

This calculator helps you calculate mass of compound based on solution concentration, volume and molecular weight in a specific solution using the formula:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

* Please check COA/MSDS for correct molecular weight.

Calculate the dilution required to prepare a stock solution.
This equation is commonly abbreviated as: C₁V₁ = C₂V₂