Bucladesine sodium (DBcAMP sodium)Cat. No. A15914
ForskolinCat. No. A12917
ISX-9Cat. No. A15774
Laduviglusib (CHIR-99021)Cat. No. A10199
GSK 1210151A (I-BET151)Cat. No. A11783
Y-27632 2HClCat. No. A11001
DFICBY Astrocyte-to-Neuron Reprogramming Kit
A six-compound small-molecule cocktail (DFICBY) for the chemical reprogramming of astrocytes into neuronal cells. One kit supplements up to 1 L of culture medium.
In Stock
Background & Details
The DFICBY Astrocyte-to-Neuron Reprogramming Kit brings together six small molecules that have been used to convert astrocytes into neuron-like cells without genetic manipulation. Bucladesine (DBcAMP) and forskolin raise cAMP signaling, and ISX-9 promotes neuronal differentiation. CHIR-99021 inhibits GSK-3, I-BET151 blocks BET bromodomain proteins to dampen the starting cell's gene program, and Y-27632 inhibits ROCK to improve cell survival during conversion.
Related cocktails have converted mouse fibroblasts into functional neurons in vitro and reprogrammed astrocytes into neurons in vivo. Each compound is supplied as a separate solid and is reconstituted in DMSO as a concentrated stock.
Description
How to use
- Briefly centrifuge each vial before opening.
- Add sterile, cell culture-grade DMSO (not included) to each vial as listed in the components table. Vortex until fully dissolved.
- Dilute the stocks into culture medium: Bucladesine sodium 1:2,000 (500 μL per 1 L); all other components 1:10,000 (100 μL each per 1 L). Sterile-filter the final medium before use.
- Store stock solutions at -20°C for up to 1 month. Aliquot to avoid repeated freeze–thaw cycles.
References
- Ma Y, et al. In vivo chemical reprogramming of astrocytes into neurons. Cell Discov. 2021;7:12.
- Li X, et al. Small-molecule-driven direct reprogramming of mouse fibroblasts into functional neurons. Cell Stem Cell. 2015;17(2):195-203.
Components
Each kit contains one vial of each of the six components below, enough to supplement up to 1 L of culture medium. The 3 Kits size contains three complete sets (up to 3 L).
| Component | Cat. No. | CAS | MW | Target | Amount | DMSO to add | Stock | Working conc. |
|---|---|---|---|---|---|---|---|---|
| Bucladesine sodium (DBcAMP sodium) | A15914 | 16980-89-5 | 491.37 | cAMP analog (PKA) | 50 mg | 509 μL | 200 mM (2,000X) | 100 μM |
| Forskolin | A12917 | 66575-29-9 | 410.51 | Adenylyl cyclase activator | 5 mg | 122 μL | 100 mM (10,000X) | 10 μM |
| ISX-9 | A15774 | 832115-62-5 | 234.27 | Neuronal differentiation inducer | 10 mg | 107 μL | 400 mM (10,000X) | 40 μM |
| Laduviglusib (CHIR-99021) | A10199 | 252917-06-9 | 465.34 | GSK-3α/β | 10 mg | 107 μL | 200 mM (10,000X) | 20 μM |
| GSK 1210151A (I-BET151) | A11783 | 1300031-49-5 | 415.44 | BET bromodomains | 1 mg | 120 μL | 20 mM (10,000X) | 2 μM |
| Y-27632 2HCl | A11001 | 129830-38-2 | 320.26 | ROCK | 5 mg | 156 μL | 100 mM (10,000X) | 10 μM |
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Applications | Chemical reprogramming of astrocytes into neuronal cells. |
| Synonyms | DFICBY; DFICBY Cocktail; Astrocyte Reprogramming Kit; Astrocyte to Neuron Kit; Chemical Neuronal Reprogramming Kit |