Fig. 1 · Structure

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4-Methylumbelliferyl phosphate

Phosphatase Fluorogenic Substrate

4-Methylumbelliferyl phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Upon dephosphorylation, it produces a fluorescent product, enabling sensitive detection in enzyme activity assays. This reagent is widely utilized in research applications involving phosphatase activity, enzymatic kinetics, and biological assays requiring fluorescent readout. Additionally, it serves as a nerve agent simulant in various experimental contexts.

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Specifications

Manufacturer Adooq Bioscience
Actions Substrate
CAS Number 3368-04-5
Applications Phosphatase Fluorogenic Substrate
Formula C10H9O6P
Molecular Weight 256.15
Synonyms 4-MUP; MUP
SMILES
O=C1C=C(C)C2=CC=C(OP(O)(O)=O)C=C2O1

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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