4-Nitrophenyl β-D-cellotetraoside is a fluorescent dye that functions as a cellulose mimetic, comprising a tetramer of D-glucose units with β-1-4 glycosidic bonds. It serves as a substrate for enzymatic hydrolysis, allowing researchers to analyze the fragmentation pattern via thin-layer chromatography or by quantifying the release of 4-nitrophenol, which exhibits strong absorbance at 395 nm in alkaline solutions. This reagent is particularly useful in studies of cellulose degradation, enabling the assessment of cellulase specificity and activity.
4-Nitrophenyl β-D-cellotetraoside is a fluorescent dye that functions as a cellulose mimetic, comprising a tetramer of D-glucose units with β-1-4 glycosidic bonds. It serves as a substrate for enzymatic hydrolysis, allowing researchers to analyze the fragmentation pattern via thin-layer chromatography or by quantifying the release of 4-nitrophenol, which exhibits strong absorbance at 395 nm in alkaline solutions. This reagent is particularly useful in studies of cellulose degradation, enabling the assessment of cellulase specificity and activity.
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