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5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 25.68 mL | 128.41 mL | 256.82 mL |
| 0.5 mM | 5.14 mL | 25.68 mL | 51.36 mL |
| 1 mM | 2.57 mL | 12.84 mL | 25.68 mL |
| 5 mM | 0.51 mL | 2.57 mL | 5.14 mL |
*The above data is based on the productmolecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Catalog Num | A21116 |
|---|---|
| CAS No. | 27072-45-3 |
| Formula | C21H11NO5S |
| M. Wt | 389.38 |
| Purity | >98% |
| Synonyms | Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers |
| SMILES | OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6 |
| Storage | Store lyophilized at -20ºC, keep desiccated. |
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