Fluorescein Dyes

Cat. No.

A18557

CAS No.

60842-46-8

FITC-Dextran

FITC-Dextran is a marker consisting of coupling fluorescein-isothiocyanate to dextran. FITC-Dextrans are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used.

Cat. No.

A16107

CAS No.

3301-79-9

6-Carboxyfluorescein

Synonyms: 6-FAM

The single isomer, 6-Carboxyfluorescein, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

Cat. No.

A17197

CAS No.

1207610-07-8

LDV FITC

LDV FITC is a fluorescent ligand for α4β1 (VLA-4).

Cat. No.

A17653

CAS No.

2321-07-5

Fluorescein

Synonyms: Uranine

Fluorescein is a fluorescent tracer commonly employed in medicinal and biological research, particularly for visualizing tumor-infected tissues. As a representative green fluorophore, it serves as a versatile scaffold for the development of various green fluorescent probes. Its high solubility and brightness make it an excellent choice for applications in microscopy and flow cytometry, facilitating studies in cellular and molecular biology.

Cat. No.

A19707

CAS No.

17817-20-8

Fluorescein di(β-D-galactopyranoside)

Synonyms: FDG

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).

Cat. No.

A21122

CAS No.

92557-81-8

6-FAM SE

6-FAM SE is another isomer of carboxyfluorescein. 6-FAM, SE is mainly used in sequencing of nucleic acids and labeling nucleotides.

Cat. No.

A21290

CAS No.

92557-80-7

5-FAM SE

5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

Cat. No.

A21295

CAS No.

117548-22-8

5(6)-FAM SE

Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; FLUOS; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

Cat. No.

A24582

CAS No.

75350-46-8

Fluorescein-5-maleimide

Synonyms: N-(5-Fluoresceinyl)maleimide

Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets. Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.

Cat. No.

A24616

CAS No.

359010-69-8

Hydroxyphenyl Fluorescein

Synonyms: HPF; 3'-p-(Hydroxyphenyl) fluorescein

Hydroxyphenyl Fluorescein (HPF) functions as a stable fluorescent probe specifically targeting reactive oxygen species (ROS). This compound exhibits enhanced specificity and stability compared to traditional probes such as H2DCFDA. It generates strong green fluorescence upon reaction with intracellular peroxynitroso, making it suitable for studying oxidative stress. Hydroxyphenyl Fluorescein can be utilized in various applications, including fluorescence microscopy, high-throughput imaging, luciferase microplate assays, and flow cytometry. Excitation and emission wavelengths are 490 nm and 515 nm, respectively.

Cat. No.

A24676

CAS No.

254098-36-7

DeepRed Nucleus Dye

DeepRed Nucleus Dye is a far-red fluorescing DNA probe that effectively permeates cell membranes. It excites at 647 nm and emits fluorescence spanning from 665 nm to over 780 nm, allowing for accurate assessment of cellular DNA content. This dye is compatible with FITC and RPE-labelled antibodies, facilitating multiplexing without requiring fluorescence compensation, making it suitable for various research applications in cell biology and molecular studies.

Cat. No.

A24767

CAS No.

596-09-8

Fluorescein Diacetate

Synonyms: 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein

Fluorescein diacetate is a fluorogenic substrate that functions as a cell-permeable esterase substrate. Upon cleavage by cellular esterases, it yields fluorescein, which is highly fluorescent and allows for the visualization of viable cells. This compound is particularly useful in studies involving hGSTP1-1 and can be applied in assays for cell viability and drug response.

Cat. No.

A24773

CAS No.

518-47-8

Fluorescein sodium

Synonyms: Uranine sodium

Fluorescein sodium is a prominent fluorescent tracer employed in various medicinal and biological applications, particularly for imaging tumor-infected tissues. As a well-established green fluorophore, it serves as a foundational scaffold for the development of effective green fluorescent probes, enabling enhanced visualization and tracking in research studies. This compound's versatility makes it an essential tool for fluorescence microscopy and related imaging techniques.

Cat. No.

A24898

CAS No.

2044-85-1

2′,7′-Dichlorofluorescein diacetate

Synonyms: DCFH2-DA

2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe utilized for the detection of reactive oxygen species (ROS). It becomes fluorescent upon cleavage by intracellular esterases and subsequent oxidation, allowing for the assessment of oxidative stress levels in biological systems. This reagent is essential for studies in toxicology and cellular signaling, enabling researchers to monitor and evaluate oxidative phenomena in various experimental contexts.

Cat. No.

A24905

CAS No.

150206-05-6

5-Carboxyfluorescein diacetate N-succinimidyl ester

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell-permeable fluorescent dye with an excitation wavelength of 492 nm and an emission wavelength of 517 nm. It covalently labels intracellular proteins, enabling the tracking of lymphocyte migration and proliferation in biological research. This reagent is particularly useful for studying immune responses and cellular dynamics in various experimental settings.

Cat. No.

A24924

CAS No.

205391-02-2

DAF-2DA

Synonyms: 5,6-Diaminofluorescein diacetat

DAF-2DA (5,6-Diaminofluorescein diacetate) is a fluorescent probe designed for the selective detection of nitric oxide (NO) in biological samples. Upon reaction with NO, DAF-2DA is converted into a highly fluorescent compound, allowing for quantitative measurement of NO levels. This reagent is widely utilized in cellular and physiological studies to investigate NO signaling pathways and its role in various biological processes.

Cat. No.

A24933

CAS No.

127770-45-0

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is a cell-permeant fluorescent indicator that targets reactive oxygen species (ROS) within cells. This reagent is particularly useful for monitoring ROS generation in human neuronal-glial (HNG) cells during primary co-culture experiments. Its ability to penetrate cellular membranes makes it a valuable tool for evaluating oxidative stress and related biological processes.

Cat. No.

A24936

CAS No.

107827-77-0

5-Dodecanoylaminofluorescein

5-Dodecanoylaminofluorescein is a lipophilic fluorescent probe that serves as a free fatty acid conjugate of fluorescein. This compound is instrumental in studies of membrane fluidity and the determination of critical micelle concentrations of detergents. Additionally, it can be utilized in the synthesis of hydrophobic nanospheres, enhancing applications in drug delivery systems.

Cat. No.

A24954

CAS No.

76-54-0

2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein is a potent fluorescent probe primarily used for the detection of oxidative stress by quantifying reactive oxygen species (ROS) levels. It exhibits excitation and emission maxima at 503 nm and 523 nm, respectively. This compound is essential for applications in cellular biology and biochemistry, enabling researchers to study oxidative damage and its effects on biological systems.

Cat. No.

A24958

CAS No.

3348-03-6

6-CFDA

Synonyms: 6-Carboxyfluorescein diacetate

6-CFDA is a fluorescent dye commonly used in biological research for cell tracking and viability assays. It undergoes free diffusion into cells, where it is hydrolyzed by intracellular non-specific lipases to produce carboxyfluorescein, a fluorescent compound that carries an additional negative charge. This enhanced charge improves retention within cells compared to traditional fluorescein dyes, facilitating more accurate analysis of cellular processes and functions.

Cat. No.

A24963

CAS No.

124412-00-6

5-CFDA-AM

5-CFDA-AM is a fluorescent probe designed to measure enzyme activity and assess cell membrane integrity. This cell-permeable esterase substrate enters cells efficiently and is hydrolyzed by intracellular esterases to form carboxyfluorescein, which exhibits enhanced retention due to its negative charge. Its quantitative fluorescence capacity makes it a valuable tool for various biological research applications, including studies on cellular processes and viability assessments.

Cat. No.

A25322

CAS No.

1926163-65-6

Fluorescein-6-carbonyl-Asp(OMe)-Glu(OMe)-Val-DL-Asp(OMe)-fluoromethylketone

Fluorescein-6-carbonyl-Asp(OMe)-Glu(OMe)-Val-DL-Asp(OMe)-fluoromethylketone is a potent and cell-permeable inhibitor of caspase-3, acting through irreversible binding to the activated form in apoptotic cells. This reagent exhibits significant fluorescence, allowing for quantitative analysis via flow cytometry, microwell plate readers, or fluorescence microscopy. Its application is crucial for studying apoptosis and characterizing caspase-3 activity in various biological systems.

Cat. No.

A33203

CAS No.

11121-48-5

Rose Bengal

Rose Bengal is a water-soluble photosensitive dye exhibiting significant antibacterial activity through both dark-state and light-activated mechanisms. It demonstrates lethal photodynamic effects against S. carlsbergensis under aerobic light conditions while also inhibiting the growth of S. aureus in the dark. Furthermore, Rose Bengal restricts colony size in filamentous fungi and suppresses the growth of diffusive fungi. This compound is valuable for research related to infections and photodynamic therapy applications.

Cat. No.

A37468

CAS No.

2969156-01-0

Geldanamycin-FITC

Geldanamycin-FITC is a fluorescent probe that specifically targets HSP90. This compound enables the evaluation of HSP90 inhibitors through fluorescence polarization assays and can effectively detect cell surface HSP90 with an excitation/emission wavelength of 488/515 nm. It serves as a valuable tool in understanding HSP90-related biological processes and evaluating therapeutic interventions in diseases associated with this chaperone protein.

Cat. No.

A37513

CAS No.

1562024-11-6

HS-27

HS-27 is a fluorescently-tethered inhibitor targeting Heat Shock Protein 90 (Hsp90), facilitating the assessment of surface Hsp90 expression on intact tissue specimens. Comprising core elements of SNX-5422 linked through a PEG spacer to a fluorescein derivative, HS-27 selectively binds to ectopically expressed Hsp90. This reagent is particularly valuable in breast cancer research, providing a tool for applications involving visualization and treatment strategies.