5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent dye widely used as a reference dye in real-time polymerase chain reaction (qPCR). It exhibits excitation and emission maxima at 585 nm and 605 nm, respectively, allowing for efficient fluorescence detection. This dye is essential for quantitative analysis, standardizing measurements in multiplex qPCR assays, and enhancing the reliability of nucleic acid quantification.
5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent dye widely used as a reference dye in real-time polymerase chain reaction (qPCR). It exhibits excitation and emission maxima at 585 nm and 605 nm, respectively, allowing for efficient fluorescence detection. This dye is essential for quantitative analysis, standardizing measurements in multiplex qPCR assays, and enhancing the reliability of nucleic acid quantification.
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