Fig. 1 · Structure

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Acridine Orange hydrochloride

fluorescent dye

Acridine Orange hydrochloride is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.

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Biological Activity

Cellular Effect

Cell Line Activity Value Description Reference
R3230AC IC50 2.29 μM Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay [PMID: 19692249]

Specifications

Manufacturer Adooq Bioscience
CAS Number 65-61-2
Applications fluorescent dye
Formula C17H20ClN3
Molecular Weight 301.81
SMILES
CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.[H]Cl

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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