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Buffers, Detergents & Protease Inhibitors

Buffers, detergents and protease inhibitors are basic reagents for preparing, lysing and stabilizing biological samples. Zwitterionic Good's buffers such as HEPES, MOPS, PIPES and MES hold pH steady in the physiological range, while Tris serves electrophoresis and general use. Detergents such as CHAPS, polysorbate 20 and sodium dodecyl sulfate (SDS) solubilize membranes and proteins. Dithiothreitol (DTT) keeps thiol groups reduced, and ethylenediaminetetraacetic acid (EDTA) chelates divalent metal ions. Protease inhibitors such as leupeptin, aprotinin, pepstatin A and E-64, with the phosphatase inhibitor sodium orthovanadate, protect proteins in cell lysates.

Cat. No.

A44459

CAS No.

7240-90-6

X-GAL

Synonyms: BCIG

X-GAL is a chromogenic substrate for β-galactosidase that serves as a vital tool in molecular biology. Upon enzymatic cleavage by β-galactosidase, X-GAL is converted from a colorless state to a blue, insoluble product, facilitating the identification of recombinant bacterial colonies in blue-white screening applications. Additionally, X-GAL is utilized for quantifying β-galactosidase activity, making it an essential reagent in various cloning and gene expression studies.

In stock View Details

200 mg

$25.00

500 mg

$45.00

All 4 sizes
Cat. No.

A86445

CAS No.

369-07-3

ONPG

Synonyms: 2-Nitrophenyl β-D-galactopyranoside

ONPG (o-nitrophenyl-β-D-galactopyranoside) serves as a colorimetric and spectrophotometric substrate for β-galactosidase detection. This compound is hydrolyzed by β-galactosidase to release o-nitrophenol, resulting in a measurable color change, making it useful for quantifying enzyme activity in various biological assays. Its applications extend to molecular biology, microbiology, and enzymology research, facilitating studies on gene expression and metabolic functions.

In stock View Details

500 mg

$25.00

1 g

$30.00

All 3 sizes

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