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Buffers, Detergents & Protease Inhibitors

Buffers, detergents and protease inhibitors are basic reagents for preparing, lysing and stabilizing biological samples. Zwitterionic Good's buffers such as HEPES, MOPS, PIPES and MES hold pH steady in the physiological range, while Tris serves electrophoresis and general use. Detergents such as CHAPS, polysorbate 20 and sodium dodecyl sulfate (SDS) solubilize membranes and proteins. Dithiothreitol (DTT) keeps thiol groups reduced, and ethylenediaminetetraacetic acid (EDTA) chelates divalent metal ions. Protease inhibitors such as leupeptin, aprotinin, pepstatin A and E-64, with the phosphatase inhibitor sodium orthovanadate, protect proteins in cell lysates.

4 Items

  • X-GAL
    Cat. No.
    A44459
    CAS No.
    7240-90-6

    X-GAL

    Synonyms: BCIG

    X-GAL is a chromogenic substrate for β-galactosidase that serves as a vital tool in molecular biology. Upon enzymatic cleavage by β-galactosidase, X-GAL is converted from a colorless state to a blue, insoluble product, facilitating the identification of recombinant bacterial colonies in blue-white screening applications. Additionally, X-GAL is utilized for quantifying β-galactosidase activity, making it an essential reagent in various cloning and gene expression studies.

    In stock View Details
    200 mg
    $25.00
    500 mg
    $45.00
    All 4 sizes
  • IPTG
    Cat. No.
    A86381
    CAS No.
    367-93-1

    IPTG

    Synonyms: Isopropyl β-D-thiogalactoside

    IPTG (Isopropyl β-D-1-thiogalactopyranoside) acts as a molecular mimic of allolactose and serves as an inducer of the lac operon. This compound effectively triggers transcription in systems where gene expression is regulated by the lac operator, enabling controlled protein expression in biological research. IPTG is widely utilized in molecular biology and recombinant protein studies to regulate the activity of target genes within prokaryotic expression systems.

    In stock View Details
    500 mg
    $35.00
    1 g
    $40.00
    All 5 sizes
  • ONPG
    Cat. No.
    A86445
    CAS No.
    369-07-3

    ONPG

    Synonyms: 2-Nitrophenyl β-D-galactopyranoside

    ONPG (o-nitrophenyl-β-D-galactopyranoside) serves as a colorimetric and spectrophotometric substrate for β-galactosidase detection. This compound is hydrolyzed by β-galactosidase to release o-nitrophenol, resulting in a measurable color change, making it useful for quantifying enzyme activity in various biological assays. Its applications extend to molecular biology, microbiology, and enzymology research, facilitating studies on gene expression and metabolic functions.

    In stock View Details
    500 mg
    $25.00
    1 g
    $30.00
    All 3 sizes
  • IPTG hemidioxane
    Cat. No.
    A91745
    CAS No.
    1330286-44-6

    IPTG hemidioxane

    Synonyms: β-D-1-thiogalactopyranoside hemidioxane

    IPTG hemidioxane, also known as β-D-1-thiogalactopyranoside hemidioxane, serves as a potent biochemical reagent primarily utilized as an inducer of the lac operon in molecular biology research. It effectively promotes the expression of proteins in bacterial systems, enhancing the study of gene function and protein production. This reagent is essential for assays requiring controlled gene expression and is widely used in recombinant protein technology.

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    Out of stock

    All 3 sizes

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