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Cell culture and assay reagents support cell growth in vitro and the measurement of viability and proliferation. Serum-free media often add hormones such as insulin, hydrocortisone and liothyronine, the active thyroid hormone, together with L-glutamine as a nutrient. Enzymes such as trypsin and hyaluronidase help dissociate adherent cells and tissue fragments. Tetrazolium salts such as MTT and WST-8 report metabolic activity through the formation of a colored formazan. 5-Ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) label newly synthesized DNA in dividing cells. Calcein-AM and propidium iodide distinguish live from dead cells by esterase activity and membrane integrity.
Synonyms: L-Ascorbic acid 2-phosphate trisodium
2-Phospho-L-ascorbic acid trisodium acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production.
Synonyms: 2-Phospho-L-ascorbic acid; LAA2P
L-Ascorbic acid 2-phosphate is a stable derivative of vitamin C that induces osteogenic differentiation in human adipose stem cells (hASCs). It enhances collagen synthesis and promotes alkaline phosphatase (ALP) activity, along with the expression of runx2A during the differentiation process. This compound serves as a valuable supplement in culture media for studies focused on bone regeneration and related cellular processes.
Synonyms: 2-Phospho-L-ascorbic acid magnesium; LAA2P magnesium
L-Ascorbic acid 2-phosphate magnesium is a stable derivative of vitamin C that promotes osteogenic differentiation in stem cells. This compound stimulates collagen synthesis and enhances alkaline phosphatase (ALP) activity, along with the expression of runx2A in human adipose stem cells (hASCs). As a culture medium supplement, it is particularly useful for promoting osteogenic pathways in research and therapeutic applications related to bone regeneration and development.
Synonyms: 2-Phospho-L-ascorbic acid magnesium hydrate; LAA2P magnesium hydrate
L-Ascorbic acid 2-phosphate magnesium hydrate acts as an osteogenic differentiation inducer. This stable vitamin C derivative promotes collagen synthesis and enhances alkaline phosphatase (ALP) activity, as well as the expression of runx2A in human adipose stem cells (hASCs). It serves as an effective supplement in culture media to facilitate osteogenic differentiation, making it valuable for research in bone development and regenerative medicine.
