Fig. 1 · Structure
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Bromophenol blue
pH Indicator
Bromophenol blue is an acid phthalein dye that functions as a pH indicator with a transition range of pH 3 to 4.6. Its utility in electrophoresis as a tracking dye enables the visualization of biomolecules during separation processes. Additionally, Bromophenol blue serves as a sensor for quantifying compounds such as ammonia, drugs, proteins, and amino acids, with significant absorbance observed at 610 nm upon protein binding.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 115-39-9 |
| Applications | pH Indicator |
| Formula | C19H10Br4O5S |
| Molecular Weight | 669.96 |
| SMILES | O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.