Fig. 1 · Structure
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CM-H2DCFDA
ROS Detector
CM-H2DCFDA is a cell-permeable fluorescent probe designed for the detection of reactive oxygen species (ROS) within cells. Upon oxidation by ROS, this compound releases a highly fluorescent product, facilitating the measurement of cellular oxidant levels with an excitation/emission maximum of 495/530 nm. Suitable for various applications in oxidative stress research, CM-H2DCFDA provides sensitive and reliable quantification of ROS in live-cell imaging studies. Note that this reagent is light-sensitive and should be handled accordingly.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 850013-49-9 |
| Applications | ROS Detector |
| Formula | C27H19Cl3O8 |
| Molecular Weight | 577.79 |
| SMILES | CC(OC1=C(Cl)C=C2C(C3=C(C=CC(CCl)=C3)C(OC(C)=O)=O)C4=CC(Cl)=C(OC(C)=O)C=C4OC2=C1)=O.CC(OC5=C(Cl)C=C6C(C7=C(C=C(CCl)C=C7)C(OC(C)=O)=O)C8=CC(Cl)=C(OC(C)=O)C=C8OC6=C5)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.