DMT-BH1 amidite is a prominent fluorescent dye characterized by its function as a dark quencher with a broad absorption profile, peaking in the green to yellow regions of the visible spectrum. This reagent is primarily utilized in the synthesis of dual-labeled oligonucleotide probes, particularly for quantitative PCR (qPCR) applications, incorporating the 5'-quencher DusQ 1. Its ability to facilitate Förster Resonance Energy Transfer (FRET) enhances multiplexing capabilities in various assay formats, making it an essential tool for advanced genetic research.
DMT-BH1 amidite is a prominent fluorescent dye characterized by its function as a dark quencher with a broad absorption profile, peaking in the green to yellow regions of the visible spectrum. This reagent is primarily utilized in the synthesis of dual-labeled oligonucleotide probes, particularly for quantitative PCR (qPCR) applications, incorporating the 5'-quencher DusQ 1. Its ability to facilitate Förster Resonance Energy Transfer (FRET) enhances multiplexing capabilities in various assay formats, making it an essential tool for advanced genetic research.
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